Total superoxide dismutase tsod activity assay kit wst. For reliable results with wst1, certain aspects of the procedure need to be adhered to. Jan 20, 2014 superoxide dismutase activity assay kit colorimetric ab65354 is a simple and rapid assay for superoxide dismutase sod activity. Sod activity from cell lysate, plasma, serum, tissue. Superoxide dismutase sod is a primary enzyme in the defensive system against oxidative stress and is elicited to reduce the effect of superoxide radicals. Superoxide dismutase is an enzyme that alternately catalyzes the dismutation or partitioning of the superoxide o2 radical into either ordinary molecular oxygen o 2 or hydrogen peroxide h 2 o 2. A simple inexpensive colorimetric assay system for sod is reported.
The human cuzn superoxide dismutase hu cuznsod elisa quantitates hu cuznsod in human serum, plasma, amniotic fluid, urine, fetal umbilical vein blood, or cell culture medium. O 2reacts with a wst1 dye to form a colored product. Assay buffer 1 x 30 ml hematoxylin 1 vial sample dilution buffer 1 x 30 ml required materials not provided. It can be given parenterally or topically and has been used to treat conditions such as radiation cystitis 17, osteoarthritis 811, and peyronie. Superoxide dismutase sod colorimetric activity kit product.
Our oxiselect superoxide dismutase activity assay uses a xanthinexanthine oxidase xod system to. Once reconstituted, the superoxide dismutase calibrator should be aliquoted and. The superoxide dismutase sod activity assay kit provides a simple and sensitive procedure for measuring sod enzymatic activity in various sample types. Superoxide dismutase sod assay kit colorimetric lsk224100 100 tests store at 20c for research use only. Sod catalyzes the dismutation of superoxide anion to hydrogen peroxide, which is subsequently detoxified to oxygen and water by catalase or glutathione peroxidase. This assay kit is designed to measure the activity of superoxide dismutase based on the xanthine oxidase method and is suitable for.
This kit can be used to measure total superoxide dismutase tsod activity in serum, plasma, urine, cells, cell culture supernatant and tissue samples. The sensitive sod assay kit utilizes wst1 that produces a watersoluble formazan dye upon reduction with superoxide anion. Sod activity is determined by measuring the decrease in superoxide anions generated by the enzyme xanthine oxidase xo. Sod activity is assessed by measuring the dismutation of superoxide radicals generated by xanthine oxidase and hypoxanthine in. Please read these instructions carefully before beginning this assay. Sod assay kit wst allows very convenient sod assaying by utilizing dojindos. Nov, 2017 linked immunosorbent assay kit is designed for accurate quantitative measurement of human cuzn superoxide dismutase concentrations in cell culture supernatant, serum and plasma edta, citrate, heparin, amniotic fluid, fetal umbilical vein blood and urine. Superoxide dismutase sod is one of the antioxidant proteins. Superoxide dismutase assay kit ii 574601 merck millipore. The density of color is proportional to the amount of sod1 captured from the samples. Pdf one of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble. Superoxide reacts with readiview sod560 to generate a color. This assay measures the activity of sod in serum, plasma, tissue lysates, cell lysates.
The superoxide dismutase assay kit colorimetric allows for the detection and. Coefficient of variation cv intra inter at 40 u sodml sample 12% 8% recovery. This article describes an appropriate protocol that. Use the assay buffer provided in the kit or refer data sheet for instructio. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase xo activity, and is inhibited by sod. The k assay superoxide dismutase sod activity kit is designed to quantitatively measure sod activity in a variety of samples. A bovine erythrocyte sod standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Superoxide dismutase assay kit provides a convenient colorimetric means for the quantitative determination of sod enzyme activity in biological samples. The cayman chemical sod assay kit is a fast and reliable assay for the measurement of sod activity from plasma, serum, tissue homogenates, and cell lysates. Sod is responsible for catalysing the dismutation of the superoxide anion free radical o 2 into molecular oxygen and hydrogen peroxide h 2 o 2.
As shown below, xanthine is converted to superoxide radical ions o 2, uric acid and hydrogen peroxide by xanthine oxidase xo. Epiquik superoxide dismutase activityinhibition assay kit. Biovisions superoxide dismutase 1 elisa kit is a sandwich elisa assay for the quantitative measurement of sod1 in mouse serum, plasma and cell culture supernatants. To perform the assay the instructions for use provided with the kit have to be used. Cited sample type includes serum and gastric mucosa homogenates ref. Simple assay kit for quantitative measurement of superoxide dismutatse enzyme activity in biological samples.
Therefore, the inhibition activity of sod can be determined by a colorimetric method. The ht superoxide dismutase assay kit is free of interference by other catalytic activities, and is ideal for assaying sod in mammalian tissue and cell lysates in a 96 well format. Equipment required a plate reader 450 nm filter b 96well. Pdf assay of superoxide dismutase activity in a plate. Sod assay kit 19160 superoxide dismutase assay kit. Store all components at 4c, except sod standard and xanthine oxidase at. The detectx superoxide dismutase sod activity kit is designed to quantitatively measure sod activity in a variety of samples. Total superoxide dismutase tsod activity assay kit hydroxylamine method please kindly provide us the lot number on the outside of the box of the kit for more efficient service. The bioxytech assay is based on the sod mediated increase in the rate of autoxidation of 5, 6, 6a, 11btetrahydro3, 9, 10trihydroxybenzocfluorene r1. This package insert must be read in its entirety before using this product. Superoxide dismutase sod are a group of metalloenzymes, the first line of defence against reactive oxygen species rosmedicated injury. Our oxiselect superoxide dismutase activity assay uses a xanthine xanthine oxidase xod system to generate superoxide anions and a chromagen to produce a watersoluble formazan dye upon reduction by superoxide anions.
The chemical and biochemical properties of the color reagent used in this kit guarantee a very convenient application and linearity of test results over a broad range. In trevigens superoxide dismutase assay ions, generated from the conversion of xanthine to uric. Sod assay kit sufficient for 500 tests superoxide dismutase. Each trial size superoxide dismutase activity assay kit provides sufficient reagents to perform up to 20 assays, including blanks, sod standards and unknown protein samples. Superoxide dismutase is an enzyme that alternately catalyzes the dismutation or partitioning of the.
Superoxide dismutase an overview sciencedirect topics. Superoxide dismutase sod activity assay kit colorimetric lsbio. Superoxide dismutase sod colorimetric assay kit 192 tests. This sensitive sod assay kit utilizes wst1 that produces a. They are an important antioxidant defense in all cells exposed to o 2. Superoxide is produced as a byproduct of oxygen metabolism and, if not regulated, causes many types of cell damage. Detection significance according to the literature, superoxide dismutase exists in all oxygenmetabolizing cells to protect cells from excessive superoxide. Cuzn superoxide dismutase specific antibodies have been precoated onto 96well plates. The method involves generation of superoxide radical by photoreduction of riboflavin and its detection by nitrite formation from. For the measurement of total sod activity in cell lysates and tissue from any species. Take the spectrum from 240 nm to 340 nm or 250 nm to 350 nm to verify that the concentration is.
Superoxide dismutase activity assay kit colorimetric ab65354 is a robust and sensitive kit for measuring sod activity in serum, plasma. Sod does not bind to cellular membranes and is rapidly excreted from the kidney. Glutathione peroxidase, superoxide dismutase and catalase. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen. Tprs sod assay kit is a wellestablished tool for measuring sod in serum, plasma, tissue, cell lysates and other biological fluids. Sod dose response was measured with amplite colorimetric superoxide dismutase assay kit in a 96well white wallclear bottom plate with a spectrum max microplate reader molecular devices. Sod assay kits beyotime biotechnology, catalog number. Superoxide dismutase assay kit colorimetric assaybiotechnology. Unlike some other assay kits for sod, this system is minimally disturbed by trace metals. Enzychromtm superoxide dismutase assay kit esod100 quantitative colorimetric determination of sod activity description superoxide dismutases sod, ec1. Superoxide dismutase assay colorimetric assay for the quantitative determination of superoxide dismutase sod in plasma, serum, erythrocyte lysates, tissue homogenates and cell lysates. Since superoxide anions interact with the provided wst dye, yielding. The assay will exclusively recognize both natural and recombinant hu. The oxiselect superoxide dismutase activity assay is a fast and reliable kit for the measurement of.
The kit contains all reagents and solutions required for determining superoxide dismutase activity in an indirect assay method based on xanthine oxidase and a novel color reagent. Superoxide dismutase was measured using the superoxide dismu tase assay kit provided by oxis research, usa sod 525. This kit measures sod activity by utilizing tetrazolium salt, which produces a watersoluble formazan dye upon reduction with superoxide anion fig. Superoxide dismutase sod colorimetric activity kit arbor. Orgotein, an enzyme cuzn superoxide dismutase that is present in all mammalian cells, is obtained from bovine liver by several steps, including chromatography. Superoxide dismutase activity assay kit colorimetric. The sensitive sod assay kit utilizes wst1 that produces a watersoluble. Assay principle the zellx superoxide dismutase sod activity kit is designed to quantitatively measure sod activity in a variety of samples. The assay measures all types of sod activity, including cuzn, mn, and fesod types. The assay can specifically measure mitochondrial superoxide dismutase mn sod activity if kcn is used to inhibit cytosolic superoxide dismutase cuzn sod activity. Please read the complete kit insert before performing this assay.
Exposure to ethanol decreases total sod activity levels in pscs figure 108. Total superoxide dismutase tsod activity assay kit. Amplite colorimetric superoxide dismutase sod assay kit b. Superoxide dismutase assay kit ii find msds or sds, a coa, data sheets and more information. Pdf a modified spectrophotometric assay of superoxide. The kit can be used to evaluate the activity of all sod types, including cuzn, mn, and fe sod types. Sod superoxide dismutase activity assay kit colorimetric. Pdf assay of superoxide dismutase activity in a plate assay using. Total superoxide dismutase tsod activity assay kit wst1. Superoxide is produced as a byproduct of oxygen metabolism and, if. The superoxide dismutase activity kit is designed to quantitatively measure sod activity in a variety of samples. Is104 superoxide dismutase assay kit cloudclone corp. Sod activity is assessed by measuring the dismutation of superoxide radicals generated by xanthine oxidase and hypoxanthine in a convenient 96 well format. Superoxide dismutase sod is one of the most important antioxidative enzymes.
Superoxide dismutase sod activity assay nwlss supplier. Sufficient reagents for 100 experimental tests, 50 negative controls, and 50 positive controls. The superoxide dismutase sod activity assay kit utilizes wst1 that produces a watersoluble formazan dye upon reduction with superoxide anion. Recovery of spiked sod in rbc human seminal plasma was calculated as 90% using this assay. All components should be stored at 4c until the expiration date of the kit. The amplite colorimetric superoxide dismutases assay kit provides a quick and sensitive colorimetric method for measuring sod. Nwlsstm superoxide dismutase activity assay product nwksod02 for research use only. In the assay, superoxide o 2 is provided by xanthine oxidase xo catalyzed reaction. Pdf assay of superoxide dismutase activity in a plate assay. Superoxide dismutase sod colorimetric activity kit.
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